Human embryonic stem cell protocols / edited by Kursad Turksen. — 2nd ed. — New York, N.Y. : Humana Press, c2010. – (58.17/M592/v.584)

Contents

    Contents
    
    Contributors
    1. Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)
    2. Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos
    3. Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts
    4. In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol
    5. Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency
    6. Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells
    7. Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells A Straightforward， Robust， and Standardized Culture Method
    8. Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR
    9. Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications
    10. A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation
    11. Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells(hESCs) Grown Under Feeder-Free Conditions
    12. Study of Gap Junctions in Human Embryonic Stem Cells
    13. hESC Engineering by Integrase-Mediated Chromosomal Targeting 229
    14. Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts 269
    15. Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stern Cells 283
    16. Embryonic Stem Cells as a Model for Studying Melanocyte Development 301
    17. In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells 317
    18. Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds 333
    19. Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells 355
    20. Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo
    21. Genetic Manipulation of Human Embryonic Stem Cells
    22. Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media 413
    23. Human-Induced Pluripotent Stem Cells: Derivation， Propagation， and Freezing in Serum- and Feeder Layer-Free Culture Conditions 425
    Index