Bioconjugate techniques / by Greg T. Hermanson. — 2nd ed. — London : Academic, 2008. – (58.1734/H552/2nd ed.) |
Contents
CONTENTS
PART I
Bioconjugate Chemistry
1. Functional Targets
2. The Chemistry of Reactive Groups
PART II
Bioconjugate Reagents
3. Zero-Length Crosslinkers
4. Homobifunctional Crosslinkers
5. Heterobifunctional Crosslinkers
6. Trifunctional Crosslinkers
7. Dendrimers and Dendrons
8. Cleavable Reagent Systems
9. Fluorescent Probes
10. Bifunctional Chelating Agents and Radioimmunoconjugates
11. Biotinylation Reagents
12. Iodination Reagents
13. Silane Coupling Agents
14. Microparticles and Nanoparticles
15. Buckyballs, Fullerenes, and Carbon Nanotubes
16. Mass Tags and Isotope Tags
17. Chemoselective Ligation: Bioorthogonal Reagents
18. Discrete PEG Reagents
Contents Overview
PART III
Bioconjugate Applications
19. Preparation of Hapten-Carrier Immunogen Conjugates
20. Antibody Modification and Conjugation
21. Immunotoxin Conjugation Techniques
22. Preparation of Liposome Conjugates and Derivatives
23. Avidin-Biotin Systems
24. Preparation of Colloidal Gold-Labeled Proteins
25. Modification with Synthetic Polymers
26. Enzyme Modification and Conjugation
27. Nucleic Acid and 01igonucleotide Modification and Conjugation
28. Bioconjugation in the Study of Protein Interactions