Yeast functional genomics and proteomics : methods and protocols / edited by Igor Stagljar. — New York : Humana, c2009. – (58.17/M592/v.548)

Contents

    Contents
    
    1 Comparative Genome Hybridization on Tiling Microarrays to Detect Aneuploidies in Yeast
    2 Identification of Transcription Factor Targets by Phenotypic Activation and Microarray Expression Profiling in Yeast
    3 SGAM: An Array-Based Approach for High-Resolution Genetic Mapping in Saccharomyces cerevisiae
    4 Reporter-Based Synthetic Genetic Array Analysis: A Functional Genomics Approach for Investigating the Cell Cycle in Saccharomyces cerevisiae
    5 The Fidgety Yeast: Focus on High-Resolution Live Yeast Cell Microscopy 75
    6 A Genomic Approach to Yeast Chronological Aging 101
    7 Chemogenomic Approaches to Elucidation of Gene Function and Genetic Pathways 115
    8 Identification of Inhibitors of Chromatin Modifying Enzymes Using the Yeast Phenotypic Screens 145
    9 Exploiting Yeast Genetics to Inform Therapeutic Strategies for Huntington's Disease
    10 Global Proteomic Analysis of Saccharomyces cerevisiae Identifies Molecular Pathways of Histone Modifications
    11 Systematic Characterization of the Protein Interaction Network and Protein Complexes in Saccharomyces cerevisiae Using Tandem Affinity Purification and Mass Spectrometry
    12 Protein Microarrays
    13 Analysis of Protein-Protein Interactions Using Array-Based Yeast Two-Hybrid Screens 223
    14 Analysis of Membrane Protein Complexes Using the Split-Ubiquitin Membrane Yeast Two-Hybrid (MYTH) System 247
    15 Computational Analysis of the Yeast Proteome: Understanding and Exploiting Functional Specificity in Genomic Data 273