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Therapeutic antibodies : methods and protocols / edited by Antony S. Dimitrov. — [Totowa, N.J.] : Humana, c2009. – (58.17/M592/v.525) |
Contents
Contents
1 Therapeutic Antibodies: Current State and Future Trends - Is a Paradigm Change Coming Soon?
PART I: RECOMBINANT ANTIGENS
2 Preparation of Recombinant Viral Glycoproteins for Novel and Therapeutic Antibody Discovery 31
PART II: ANTIBODY LIBRARIES
3 Design of a Human Synthetic Combinatorial Library of Single-Chain Antibodies 61
4 Construction of a Human Antibody Domain (VH) Library 81
5 Generation and Selection of Rabbit Antibody Libraries by Phage Display 101
6 Construction of a Large Naive Human Phage-Displayed Fab Library Through One-Step Cloning 129
PART III: ANTIBODY DISCOVERY
7 Identification of Target and Function Specific Antibodies for Effective Drug Delivery
8 Screening of Specific Internalization Fab Fragment from Human Naive Phage Library by Combinational Bio-Panning 161
9 Competitive Antigen Panning for Selection of HIV-1 Neutralizing Human Monoclonal Antibodies Specific for gp41 175
10 Selection of Non-aggregating VH Binders from Synthetic VH Phage-Display Libraries 187
11 Isolation of Full-Length IgG Antibodies from Combinatorial Libraries Expressed in Escherichia coli 217
12 Multiplexed Flow Cytometry: High-Throughput Screening of Single-Chain Antibodies 241
13 Human Antibody Repertoires
14 Rapid Screening Platform for Stabilization of scFvs in Escherichia coli
PART IV: ANTIBODY ENGINEERING
15 In Vitro Antibody Affinity Maturation Targeting Germline Hotspots 293
16 Affinity Maturation by Phage Display
17 Production of Chimeric Heavy-Chain Antibodies 323
18 Mammalian Cell Display for Antibody Engineering 337
19 Improving Antibody Binding Affinity and Specificity for Therapeutic Development 353
20 Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy 377
21 Deimmunization of Monoclonal Antibodies 405
22 Anti-CD22 Onconase: Preparation and Characterization 425
23 Antibody-Cytotoxic Agent Conjugates: Preparation and Characterization 445
PART V: ANTIBODY PRECLINICAL DEVELOPMENT
24 High-Level Production of a Humanized ImmunoRNase Fusion Protein from Stably Transfected Myeloma Cells 471
25 Antibody Fragment Expression and Purification 491
26 Scaling-Up and Production of Therapeutic Antibodies for Preclinical Studies 499
27 Antibody Production by the Gram-Positive Bacterium Bacillus megaterium 509
28 Neutralization of HIV by Antibodies
29 Dynamics of Antibody Domains Studied by Solution NMR 533
30 In Vivo Method for Establishing Synergy Between Antibodies to Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor-2 545
31 Passive Immunization with Human Neutralizing Monoclonal Antibodies Against HIV-1 in Macaque Models: Experimental Approaches 559
PART VI ANTIBODY SEQUENCE ANALYSIS
32 Organizing， Exploring， and Analyzing Antibody Sequence Data: The Case for Relational-Database Managers 569
Index
