Hepatocyte transplantation : methods and protocols / edited by Anil Dhawan, Robin D. Hughes. — Totowa : Humana, c2009. – (58.17/M592/v.481)

Contents

    Contents
    
    Preface
    Acknowledgements
    Contributors
    Color Plates
    1 Human Hepatocyte Transplantation Overview
    2 Isolation of Human Hepatocytes
    3 An Optimised Method for Cryopreservation of Human Hepatocytes 25
    4 Liver Cell Culture Techniques
    5 In Vitro Assays for Induction of Drug Metabolism
    6 Hepatocyte Apoptosis 59
    7 Small Animal Models of Hepatocyte Transplantation 75
    8 Hepatocyte Transplantation Techniques: Large Animal Models 83
    9 Cell Transplant Techniques: Engraftment Detection of Cells 97
    10 Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation
    11 Ex Vivo Gene Transfer into Hepatocytes 117
    12 Sources of Adult Hepatic Stem Cells: Haematopoietic 141
    13 Production of Hepatocyte-Like Cells from Human Amnion 155
    14 Generation of Hepatocytes from Human Embryonic Stem Cells 169
    15 Isolation， In Vitro Cultivation and Characterisation of Foetal Liver Cells 181
    16 Human Intrahepatic Biliary Epithelial Cell Lineages: Studies In Vitro 193
    17 Liver Cell Labelling with MRI Contrast Agents 207
    18 Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory 221
    Index