Extracellular matrix protocols / edited by Sharona Even-Ram and Vira V. Artym. — 2nd ed. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.522)

Contents

    Contents
    
    Preface
    Contributors
    PART I: MOLECULAR BIOLOGY
    1 Retroviral Delivery of ECM Genes
    2 Tissue-Specific KO of ECM Proteins
    3 Recombinant Collagen Trimers from Insect Cells and Yeast
    4 Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag
    5 Preparation of Recombinant Fibronectin Fragments for Functional and Structural Studies
    PART II: BIOCHEMICAL AND BIOPHYSICAL ANALYSIS
    6 Quantitative Determination of Collagen Cross-links
    7 ECM Macromolecules: Height-Mapping and Nano-Mechanics Using Atomic Force Microscopy
    8 Atomic Force Microscopy Measurements of Intermolecular Binding Forces
    9 Mass-Mapping ofECM Macromolecules by Scanning Transmission Electron Microscopy
    10 Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry
    11 ECM Macromolccules: Rotary Shadowing and Transmission Electron Microscopy
    12 Using Sell-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates
    13 Solid Phase Assays for Studying ECM Protein-Protein Interactions
    PART III: CELL BIOLOGY ASSAYS
    14 Cell Adhesion Assays
    15 ECM Degradation Assays for Analyzing Local Cell Invasion
    16 Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration
    17 Fibrin Gel Model for Assessment of Cellular Contractility
    18 Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis)
    19 Stromagenesis During Tumorigenesis: Characterization of Tumor-Associated Fibroblasts and Stroma-Derivcd 3D Matrices
    PART IV: ORGAN MODELS
    20 Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
    21 ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gone Expression During Submandibular Gland Development
    22 Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice
    PART V: TISSUE ENGINEERING
    23 Characterizing ECM Production by Ceils Encapsulated in Hydrogels
    24 Tissue Enginecring and Cell-Populated Collagen Matrices
    Index