Kidney research : experimental protocols / edited by Tim D. Hewitson, Gavin J. Becker. — New York, N.Y. : Humana, c2009. – (58.17/M592/v.466)

Contents

    Contents
    
    Preface v
    Contributors ix
    List of Color Plates xiii
    Part I Isolation and Propagation of Cell Populations
    1 Isolation and Propagation of Glomerular Mesangial Cells
    2 Isolation and Primary Culture of Human Proximal Tubule Cells
    3 Propagation and Culture of Renal Fibroblasts
    Part II Animal Models
    4 Small Animal Models of Kidney Disease: A Review
    Part III Techniques and Applications
    5 Measurement of Glomerular Filtration Rate in Conscious Mice
    6 Laser Capture Microdissection of Kidney Tissue
    7 Quantitative Gene Expression Analysis in Kidney Tissues
    8 In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy 109
    9 Using In Situ Hybridization to Localize Renal Gene Expression in Tissue Sections 119
    10 Immuno and Lectin Histochemistry for Renal Light Microscopy 133
    11 Immuno and Lectin Histochemistry for Renal Electron Microscopy 149
    12 Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia 161
    13 Identification of Apoptosis in Kidney Tissue Sections 175
    14 Cell-Populated Floating collagen lattices: an in vitro model of parenchymal contraction
    15 Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells 205
    16 Determination of Collagen Content， Concentration， and Sub-types in Kidney Tissue 223
    17 SELDI-TOF mass spectrometry-based protein profiling of kidney tissue
    18 In vivo transfer of small interfering RNA or small hairpin RNA targeting Glomeruli