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Plant pathology : techniques and protocols / edited by Robert Burns. — New York : Humana Press, c2009. – (58.17/M592/v.508) |
Contents
Contents
Preface
Contributors
1. Bioassay for Diagnosis of Plant Viruses
2. Development of Enzyme Linked， Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection
3. Immunisation Strategies for Antibody Production
4. Preparation of Immunogens and Production of Antibodies
5. Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience
6. Production of Monoclonal Antibodies to Plant Pathogens 63
7. Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp， sepedonicus) 75
8. Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Ralstonia solanacearum ) 89
9. Detection of Four Major Bacterial Potato Pathogens 101
10. Erwinia amylovora : Modern Methods for Detection and Differentiation 115
11. The Use of Fluorescent In Situ Hybridization in Plant Fungal Identification and Genotyping 131
12. Use of Molecular Methods for the Detection of Fungal Spores 147
13. Identification of Phytophthora fragariae var. rubi by PCR 161
14. Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani 171
15. Immunocapture-PCR for Plant Virus Detection 183
16. Multiplex Polymerase Chain Reaction (PCR) and Real-Time Multiplex PCR for the Simultaneous Detection of Plant Viruses 193
17. Fluorescent-Based Techniques for Viral Detection， Quantification， and Characterization
18. Analysis of Population Structures of Viral Isolates Using Single-Strand Conformation Polymorphism Method 235
19. Direct Detection of Plant Viruses in Potato Tubers Using Real-Time PCR
20. An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil
21. Detection of Phytoplasmas of Temperate Fruit Trees 267
22. PCR Detection of Potato Cyst Nematode
23. Potato Cultivar Genome Analysis 295
24. Barley Variety Identification Using SSRs 309
Index 319
