Methods in cell biology. v.90, Methods in nano cell biology / edited by Bhanu P. Jena. — Amsterdam : Elsevier, c2008. – (58.1574/M592/v.90)

Contents

    CONTENTS
    
    Contributors
    Preface
    1. Extracellular Dynamics at nm Resolution in Live Cells
     I. Introduction
     II. Materials and Methods
     III. Conclusion
    2. Intracellular Organelle Dynamics at nm Resolution
     I. Introduction
     II. Materials and Methods
     III. Discussion
    3. Atomic Force Microscopy as a Tool to Study the Proteasome Assemblies
     I. Introduction
     II. Rationale
     III. Methods
     IV. Discussion
     V. Summary
    4. Biomineralization at the Nanoscale: Learning from Diatoms
     I. Introduction to Nanotechnology
     II. Introduction to Diatoms
     III. Investigating Diatom Mesoscale Silica Formation
     IV. Nanoscale Imaging of Living Diatoms
     V. Nanoscale Elasticity Measurements
     VI. Examination of Diatom Valves
     VII. Examination of Diatom Girdle Bands
     VIII. Examination of Diatom Setae
     IX. Future Directions
    5. Quantitative Phase Imaging of Nanoscale Cell Structure and Dynamics
     I. Introduction and Rationale
     II. Methods
     III. Applications of QPI
     IV. Summary and Outlook
    6. Fourier Imaging Correlation Spectroscopy for Cellular Structure-Function
     I. Introduction and Background
     II．Theoretical Descriptions of FICS
     III. FICS Optical Layout
     IV. Examples of FICS Applications
     V. Conclusions
    7. Nanobiology of the Primary Cilium--Paradigm of a Multifunctional Nanomachine Complex
     I. Introduction
     II. Self-Assembly
     III. Ciliogenesis
     IV. Elucidating the Functional Role of Primary Cilia
     V. The Medical Consequences of Aciliogenesis--A Veritable Pandora's Box
     VI. Concluding Remarks
    8. Assembly and Disassembly of SNAREs in Membrane Fusion
     I. Introduction
     II. Materials and Methods
     III. Discussion
    9. Understanding Membrane Fusion: Combining Experimental and Simulation Studies
     I. Introduction
     II. Materials and Methods
     III. Discussion
    10. Structure and Dynamics of Metalloproteins in Live Cells
     I. Introduction
     II. Rationale
     III. Methods
     IV. Materials
     V. Discussion
     VI. Summary
    11. Light-Activated Ion Channels for Remote Control of Neural Activity
     I. Introduction
     II. Discussion III. Methods IV. Conclusion
    12. Molecular Modeling and Simulation Studies of Ion Channel Structures， Dynamics and Mechanisms
     I. Introduction
     II. Homology-Based Structure Prediction of Transmembrane Proteins
     III. PB Profiles for the Energetics of an Ion in a Channel
     IV. MD Simulations
     V. Free Energy Methods
     VI. Summary
    13. Nano-Scale Imaging and Dynamics of Amylin-Membrane Interactions and Its Implication in Type II Diabetes Mellitus
     I. Introduction
     II. Materials and Methods
     III. Results and Discussion
     IV. Sunnnary
    14. Real Time Investigation of Protein Folding， Structure， and Dynamics in Living Cells
     I. Introduction
     II. Rationale
     III. Methods and Materials
     IV. Summary
    15. Structural Basis of Human High-density Lipoprotein Formation and Assembly at Sub nanometer Resolution
     I. Introduction
     II. Rationale
     III. Methods and Materials
     IV. Discussion
     V. Summary
    16. Nano-scate Characterization of the Dynamics of the Chloroplast Toc Translocon
     I. Introduction
     II. In organeller Chloroplast hnport and Binding Assays
     III. Toc Translocon Dynamics
     IV. Activity and Enzymology of Toc GTPases
     V. Molecular Modeling of Toc Proteins
     VI. Conclusions and Perspectives
    17. A System Biology Approach to Understand Functional Activity of Cell Communication Systems
     I. Introduction
     II. Methods
     III. Materials
     IV. Discussion
    18. THz Investigations of Condensed Phase Biomolecular Systems
     I. Introduction
     II. Instrumentation
     III. Theory
     IV. Dipeptides Nanotubes
    19. Combined Single-Molecule Electrical Recording and Single-Molecule Spectroscopy Studies of Ion Channel Conformational Dynamics
     I. Introduction
     II. Development of Simultaneous Single-Molecule Fluorescence Spectroscopy and Patch-Clamp Electrical Recordings
     III. Summary and Prospects
    Index