SARS- and other coronaviruses : laboratory protocols / edited by Dave Cavanagh. — N.Y. : Humana Press, c2008. – (58.17/M592/v.454)

Contents

    Contents
    
    Preface v
    Contributors xi
    Extra Notes From the Editor xv
    PART I DETECTION AND DISCOVERY OF CORONAVIRUSES
    1 A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses 3
    2 Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions 13
    3 Detection and Sequence Characterization of the 3'-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m 27
    4 RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken， Turkey， Pheasant) and in a Parrot 35
    5 Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains 43
    6 Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions 61
    7 Detection of New Viruses by VIDISCA
    PART II ISOLATION， GROWTH， TITRATION， AND PURIFICATION OF CORONAVIRUSES
    8 Titration of Human Coronaviruses， HCoV-229E and HCoV-OC43， by an Indirect Immunoperoxidase Assay 93
    9 The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation， Propagation， and Titration
    10 Isolation and Propagation of Coronaviruses in Embryonated Eggs 109
    11 Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen
    PART III STRUCTURE OF CORONAVIRUSES ANALYZED BY ELECTRON MICROSCOPY
    12 Purification and Electron Cryomicroscopy of Coronavirus Particles
    PART IV EXPRESSION OF CORONAVIRUS PROTEINS AND CRYSTALLIZATION
    13 Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization 139
    PART V RAISING ANTIBODIES TO CORONAVIRUS PROTEINS
    14 Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus 163
    15 Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus 191
    16 Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins 205
    PART VI MANIPULATING THE GENOMES OF CORONAVIRUSES
    17 Manipulation of the Coronavirus Genome Using Targeted RNA Recombination with Interspecies Chimeric Coronaviruses 229
    18 Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs 237
    19 Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses 255
    20 Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes 275
    21 Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome 293
    PART VII IDENTIFYING CORONAVIRUS RECEPTORS
    22 Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes 319
    23 Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins 331