Germline stem cells / edited by Steven X. Hou, Shree Ram Singh. — Totowa, N.J. : Humana Press, c2008. – (58.17/M592/v.450)

Contents

    Contents
    
    Preface v
    Contributors ix
    Part I Identification and Regulation of Germline Stem Cells in Model Organisms
    1 The Development of Germline Stem Cells in Drosophila
    2 Analysis of the C. elegans Germline Stem Cell Region 27
    3 Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell 45
    4 Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells 61
    5 Structural Polarity and Dynamics of Male Germline Stem Ceils in an Insect (Milkweed Bug Oncopeltusfasciatus) 71
    6 High-Resolution Light Microscopic Characterization of Spermatogonia
    7 Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes 109
    8 Epigenetic Control in Male Germ Cells: A Transillumination-Assisted Microdissection Method for the Analysis of Developmentally Regulated Events 119
    9 GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro 127
    Part II In Vitro Culture and Applications of Germline Stem Cells
    10 Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts 139
    11 Spermatogonial Stem Cell Transplantation， Testicular Function， and Restoration of Male Fertility in Mice 149
    12 Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture 163
    13 Deriving Mouse Spermatogonial Stem Cell Lines 181
    14 Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes 193
    15 Testicular Germ Cell Tumors in Mice: New Ways to Study a Genetically Complex Trait 211
    16 Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries 233
    Index 267