Cryopreservation and freeze-drying protocols / edited by John G. Day, Glyn N. Stacey. — 2nd ed. — Totowa, N.J. : Humana Press, c2007.—(58.17/M592/v.368)

Contents

    Contents
    
    Preface
    Contributors
    Glossary of Specialized Terms
    1 Long-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers 1
    2 The Principles of Freeze-Drying 15
    3 Principles of Cryopreservation 39
    4 Lyophilization of Proteins 59
    5 Vacuum-Drying and Cryopreservation of Prokaryotes 73
    6 Freeze-Drying of Yeast Cultures 99
    7 Cryopreservation of Yeast Cultures 109
    8 Freeze-Drying Fungi Using a Shelf Freeze-Drier 119
    9 Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying 127
    10 Cryopreservation of Microalgae and Cyanobacteria 141
    11 Cryopreservation of Plant Cell Suspensions 153
    12 Cryopreservation of Shoot Tips and Meristems
    13 Cryopreservation of Desiccation-Tolerant Seeds
    14 Cryopreservation of Fish Sperm
    15 Cryopreservation of Avian Spermatozoa 219
    16 Cryopreservation of Animal and Human Cell Lines 227
    17 Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use 237
    18 Cryopreservation of Human Embryonic Stem Cell Lines 261
    19 Cryopreservation of Primary Animal Cell Cultures 271
    20 Cryopreservation of Red Blood Cells and Platelets 283
    21 Cryopreservation of Mammalian Semen 303
    22 Cryopreservation of Mammalian Oocytes 313
    23 Cryopreservation of Mammalian Embryos 325