PRINS and in situ PCR protocols / edited by Franck Pellestor. — 2nd ed. — Totowa, New Jersey : Humana Press, c2006.—(58.17/M592/v.334)

Contents

            Contents
    
    Dedication
    Preface
    Acknowledgments
    Contributors
    List of Color Pages
    PART I. BASIC METHODOLOGY
    1. New Rapid Multicolor PRINS Protocol
    2. PRINS Combined With Peptide Nucleic Acid Labeling
    3. PRINS Combined With Indirect Immunofluorescence
    4. PRINS for the Detection of Unique Sequences
    5. Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS)
    6. Analysis of Sperm Aneuploidy by PRINS 49
    7. In Situ PCR Protocols 61
    PART II. RESEARCH AND CLINICAL APPLICATIONS
    8. Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS 81
    9. PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei 89
    10. PRINS for the Detection of Gene Deletions in Cancer
    11. Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene
    12. Brain Tissue Preparations for Chromosomal PRINS Labeling 123
    13. PRINS on Plant Chromosomes 133
    14. Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood 141
    15. PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos 151
    16. Microdissection With PCR In Situ 161
    17. Application of Reverse Transcription In Situ PCR in Cancer Analysis 169
    18. In Situ Reverse Transcription PCR on Plant Tissues 181
    19. In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection 199
    20. Development and Adaptation of the PRINS Technology: An Overview 211
    21. Latest Developments in In Situ PCR 221
    Index 241