Dictyostelium discoideum protocols /edited by Ludwig Eichinger, Francisco Rivero. --Humana Press, 2006. –(58.17/M592/v.346) |
Contents
Contents:
1. The secret lives of dictyostelium 3
2. The genome of dictyostelium discoideum 15
3. The cDNA sequencing project 31
4. dictybase and the dicty stock center 51
5. Analysis of gene expression using cDNA microarrays 75
6. Proteomic analysis of dictyostelium discoideum 95
7. Cultivation, spore production and mating 113
8. Parasexual genetics using axenic cells 125
9. Slug phototaxis, thermotaxis, and spontaneous turning behavior 137
10. Purification techniques of subcellular compartments for analytical and preparative purposes 171
11. Generation of multiple knockout mutants using the cre-loxp system 187
12. Restriction enzyme-mediated integration (REMI) mutagenesis 201
13. RNA interference and antisense-mediated gene silencing in dictystelium
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