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Dictyostelium discoideum protocols /edited by Ludwig Eichinger, Francisco Rivero. --Humana Press, 2006. –(58.17/M592/v.346)

Contents

 

Contents:

1.       The secret lives of dictyostelium  3

2.       The genome of dictyostelium discoideum  15

3.       The cDNA sequencing project  31

4.       dictybase and the dicty stock center  51

5.       Analysis of gene expression using cDNA microarrays  75

6.       Proteomic analysis of dictyostelium discoideum  95

7.       Cultivation, spore production and mating  113

8.       Parasexual genetics using axenic cells  125

9.       Slug phototaxis, thermotaxis, and spontaneous turning behavior  137

10.   Purification techniques of subcellular compartments for analytical and preparative purposes  171

11.   Generation of multiple knockout mutants using the cre-loxp system  187

12.   Restriction enzyme-mediated integration (REMI) mutagenesis  201

13.   RNA interference and antisense-mediated gene silencing in dictystelium

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