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High content screening :a powerful approach to systems cell biology and drug discovery /edited by D. Lansing Taylor, Jeffrey R. Haskins, Kenneth A. Giuliano. --Humana Press,2007. –(58.17/M592/v.356) |
Contents
Contents:
1. Past, present, and futuree of high content screening and the field of cellomics 3
2. A pharmacyeutical company user’s perspective on the potential of high content screening in drug discovery 19
3. Linking microscopy and high content screening in large-scale biomedical research 33
4. Requirement, features, and performance of high content screening platforms 41
5. Characteristics and value of directed algorithms in high content screening 63
6. Characteristics and value of machine learning for imaging in high content screening 83
7. Tools for quantitative and validated measurements of cells 95
8. Automated cell plating and sample treatments for fixed cells in high content assays 109
9. Differentiating primary human cells in rapid-throughput discovery applications 121
10. Use of the cellcardTM System for analyzing multiple cell types in parallel 129
11. Reagents to measure and manipulate cell functions 141
12. Fluorescent proteins and engineered cell lines 165
13. Optimizing the integration of immuneoreagents and fluorescent probes for multiplexed high contents screening assays 189
14. The halotagTM: a novel technology for cell imaging and protein analysis 195
15. Protein labeling with flash and reash 209
16. Exploiting network biology to improve drug discovery 221
17. Physiological indicators of cell function 233
18. The use of siRNA to validate immunofluorescence studies 245
19. Caged substrates applied to high content screening: an introduction with an eye to the futuree 253
20. Overview of informatics for high content screening 269
21. Large-scale data management for high content screening 281
22. An integrated biomedical knowledge extraction and analysis platform: using federated search and document clustering technology 293
23. Visualization of high content screening data 301
24. Pathway maping tools for analysis of high content data 319
25. Systems biology in cancer research: genomics to cellomics 353
26. Target validation in drug discovery 367
27. High content screening as a screening tool in drug discovery 379
28. Discovery of protein Kinase Phosphatase inhibitors 389
29. High content translocation assays for pathway profiling 401
30. In vitro cytotoxicity assessment 415
31. Neurite outgrowth in retinal ganglion cell culture 427
