Xenopus protocols :cell biology and signal transduction /edited by X. Johne Liu --Humana Press, 2006. –(58.17/M592/v.322)

Contents

Contents:

1.       Resources for Genetic and Genomic Studies of Xenopus  1

2.       The Physiology of the Xenopus laevis Oary  17

3.       Oxcyte Isolation and Enucleation  31

4.       Xenopus tropicalis Oocytes: More than just a beautiful genome  43

5.       Oocyte expression with injection of purified T7 RNA Polymerase  55

6.       Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy  69

7.       Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research  87

8.       Imaging Ca²⁺Signals in Xenopus Oocytes  103

9.       Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs  121

10.   Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes  139

11.   Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes  149

12.   Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles  165

13.   Cytoplasmic mRNA Polyadeylation and Translation Assays  183

14.   Xenopus Egg Extracts: Amodel System to study Proprotein Convertases  199

15.   Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay  213

16.   Ubiqutin-Mediated Protein Degradation in Xenopus Egg Extracts  223

17.   Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms  235

18.   Use of Xenopus Laevis Oocyte Nuclein and Nuclear Envelopes in Nucleocytoplasmic Transport Studies  259

19.   Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy  273

20.   In Vitro Study of  Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts  289

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