Signal transduction protocols /edited by Robert C. Dickson and Michael D. Mendenhall. -- 2nd ed. -- (Methods in molecular biology ; v. 284). -- Humana Press, 2004. -- (58.17/M592/v.284)

Contents:

1. Making Protein Immunoprecipitates  1

2. Signal Transduction Inhibitors in Cellular Function  15

3. Two-Dimensional Gel Electrophoresis for the Indentification of Signaling Targets  37

4. A High-Throughput Mammalina Cell-Based Transient Transfection Assay  51

5. Determining Protein Half-Lives  67

6. Assaying Protein Kinase Activity  79

7. Comparative Phosphorylation site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based approach  91

8. Studies of Calmodulin-Dependent Regulation  111

9. Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation  129

10. Characterization of Protein-DNA Association In Vivo by Cheomatin Immunoprecipitation  147

11. Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins  163

12. Nucleocytoplasmic glycosylation, O-GlcNAc: Indentification and Site Mapping  175

13. Techniques in Protein Methylation  195

14. Assaying Lipid Phosphate Phosphatease Activities  209

15. Assaying Phosphoinositide Phosphatases  217

16. Assaying Phospholipase A(2) Activity  229

17. Measurement and Immunogluorescence of Cellular Phosphoinositides  243

18. Measuring Dynamic changes in cAMP Using Fluorescence Resonance Energy Transfer  259

19. In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET  271

20. Revealing Protein Dynamics by Photobleaching Techniques  287

21. Assaying Cytochrome c Translocation During Apoptosis  307