Nitric oxide protocols /edited by Aviv Hassid. -- 2nd ed. -- (Methods in molecular biology ; v.279). -- Humana Press, 2004. -- (58.17/M592/v.279)

Contents:

1. Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO  1

2. High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples  9

3. Nitrite and Nitrate Measuremants in Human Urine by Capillary Electrophoresis  21

4. In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode  35

5. Measurement of Exhaled Nitric Ocide  45

6. Nitric Oxide Imaging in Neurons Using Confocal Microscopy  69

7. Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry  81

8. A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts  105

9. Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization  113

10. Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase  125

11. Dioxygen-Dependent Metabolism of Nitric Oxide  133

12. Proteomic Method for Identification of Tyrosine-Nitrated Proteins  151

13. Immunohistochemical Detection of S-Nitrosylated Proteins  167

14. Gene Therapy Tehcniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction  173

15. Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats  187

16. Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase  201

17. gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors  213

18. Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer  225