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Mammalian artificial chromosomes : methods and protocols / edited by Vittorio Sgaramella and Sandro Eridani. -- (Methods in molecular biology ; v. 240). -- Humana Press, 2004. -- 58.17/M592/v.240

Contents

Contents:

1. From Natural to Artificial Chromosomes: an Overview  1

2. Methodologies Applied to Domestic Animal Chromosomes  15

3. Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes  35

4. Genomic Imprinting and its Effects on Genes and Chromosomes in Mammals  53

5. Centromeres and Neocentromeres  77

6. Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins  105

7. Telomere Length analysis and In Vitro Telomerase Assay  123

8. Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice  147

9. Chromosome-Based Vectors for Mammalian Cells: an Overiew  167

10. Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor  187

11. Use of Natural and Artificial Chromosomes Vectors for Animal Transgenesis  207

12. Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique  227

13. Modification of Human Bacterial Artificial chromosome Clones for functional Studies and Therapeutic Applications  243