Mammalian artificial chromosomes : methods and protocols / edited by Vittorio Sgaramella and Sandro Eridani. -- (Methods in molecular biology ; v. 240). -- Humana Press, 2004. -- 58.17/M592/v.240 |
Contents
Contents: 1. From Natural to Artificial Chromosomes: an Overview 1 2. Methodologies Applied to Domestic Animal Chromosomes 15 3. Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes 35 4. Genomic Imprinting and its Effects on Genes and Chromosomes in Mammals 53 5. Centromeres and Neocentromeres 77 6. Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins 105 7. Telomere Length analysis and In Vitro Telomerase Assay 123 8. Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice 147 9. Chromosome-Based Vectors for Mammalian Cells: an Overiew 167 10. Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor 187 11. Use of Natural and Artificial Chromosomes Vectors for Animal Transgenesis 207 12. Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique 227 13. Modification of Human Bacterial Artificial chromosome Clones for functional Studies and Therapeutic Applications 243 |