G Protein signaling : methods and protocols / edited by Alan V. Smrcka. -- (Methods in molecular biology ; v. 237). -- Humana Press, 2004. -- 58.17/M592/v.237

Contents:

1. Purification of G Protein Subutein and G Protein Effectors  3

2. Assays for G Protein-Dependent Effector Activation in Vitro  67

3. Assays for G Protein-Coupled Receptor-Mediated and G Protein Subunit-Mediated Effector Activation in Intact Cells  89

4. Receptor Mechanisms, Activity, and Localization  105

5. Disruption of Endogenous G Protein-Signaling Systems to Understand the Role of G Protein Subunits in Intact Systems  153

6. Posttranslational Modifications of Regulators of G Protein Signaling  195

7. Use of GFP-Tagged G Proteins and Effectors in Intact Cells  223