Chemistry biology : a practical course / by Herbert Waldmann, Petra Janning. -- Wiley-VCH, 2004. -- 58.173/W164

Contents

Contents:

1. Introduction: Chemical Biology - A New Science at the Crossroads of Chemistry and Biology  1

2. DNA Synthesis and DNA Hybridization  23

3. Doubly-labeled Peptide Nucleic Acids (PNAs) as Probes for the Detection of DNA Point Mutations  35

4. Synthesis and Characterization of a Covalent Oligonucleotide-Streptavidin conjugate and its Application in DNA-directed Immobilization (DDI)  45

5. Solid Phase Synthesis of Peptides: Bradykinin Analogs and the Evaluation of Calcium Mobilization in PC-12 Cells  61

6. In Sillico Protein Ligand Design  75

7. Lipidation of Proteins and Peptides: Farnesylation of the Ras Protein  107

8. Insertion of Lipidated Peptides into Model Mambranes  123

9. Isolation of Potato Phosphorylase and Enzymatic Synthesis of Amylose  137

10. Proteome Analysis: Identification of Proteins Isolated from Yeast  145

11. Lectins: Determination of the Sugar Specificity of Jacalin by a Sugar-Lectin Binding Assay (SLBA)  161

12. Combinatorial Synthesis and Genetic Algorithm  171

13. Solid Phase Synthesis of an Antibiotic  181