Germ Cell Protocols. v.2: molecular embryo analysis, live imaging, transgenesis, and cloning / edited by Heide Schatten. -- (Methods in molecular biology ; v. 254). -- Humana press, 2004

Contents

索书号: 58.17/M592/v.254

1. Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos  1

2. Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans  35

3. cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development  49

4. PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells: Cloning Uniquely Expressed RNAs from Rare Cells  67

5. Differentiating Embryonic Stem Cells into Embryoid Bodies  79

6. The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation  99

7. Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos  113

8. Dual-Channel Confocal Rating of Calcium Dynamics in Living Eggs and Oocyres  137

9. A Method for Producing Cloned Pigs Using Somatic Cells as Donors  149

10. Pronuclear Microinjection of Mouse Zygotes  165

11. Parthenogenesis and Nuclear Transfer in Rabbit Oocytes  195

12. Nuclear Transfer in Cattle  213

13. In Vitro Fertilization and Embryo Transfer in Felids  227

14. Production of Chimeric Chickens  245

15. Production of Haploid and Diploid Androgenetic Zebrafish  255

16. Construction and Detection of Fluorescent, Germline Transgenic Zebrafish  271

17. Production of Zebrafish Germline from Cultured Cells  289

18. Studing Nuclear Reprogramming with Cell Hybrids  301

19. Cytoplasmic Transfer in Mammalian Eggs and Embryos  313

20. Conventional Technologies for Cryopreservation of Human Oocytes and Embryos  325

21. Vritifying and Warming of Human Oocytes, Embryos, and Blastocysts: Vitroification Procedures as an Alternative to Conventional Cryopreservation  345

22. Microfluidic Removal of Cumulus Cells from Mammalian Zygotes  365

23. Mammalian Embryo Culture in a Microfluidic Device  375

24. application of Alginate Gels to the Study of Mammalian Development  383

25. Using TUNEL in Combination with an Active Caspase-3 Immunoassay to identify Cells Undergoing Apoptosis in Preimplantation Mammalian Embryos  393