Gene Biotechnology / by William Wu ... [et. al.]. -- 2nd ed. -- CRC, 2004

Contents

1. Strategies for Novel Research Projects  1

2. Rapid Isolation of Specific cDNAs or Genes by PCR  15

3. Construction and Screening of Subtracted and Complete Expression cDNA Libraries  31

4. Subcloning of Genes or DNA Fragments  73

5. Nonisotopic and Isotopic DNA or RNA Sequencing  99

6. Information Superhighway and Computer Databases of Nucleic Acids and Proteins  139

7. Characterization of DNA or Genes by Southern Blot Hybridization  165

8. Gene Overexpression by Sense RNA in Mammalian Systems  193

9. Gene Underexpression in Cultured Cells and Animals by Antisecse DNA and RNA Strategies  223

10. Analysis of Gene Expression at the Functional Genomic Level  247

11. Analysis of Gene Expression at the Proteomic Level  265

12. Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH)  287

13. Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH)  311

14. In Situ PCR Hybridization of Low Copy Genes and  in Situ RT-PCR Detection of Low Abumdance mRNAs  323

15. Isolation and Characterization of Genes from Genomic DNA Libraries  341

16. Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression  365

17. New Strategies for Gene Knockout  375

18. Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells  409

19. Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR  445

20. High-Throughput Analysis of Gene Expression by Cutting-Edge Technology- DNA Microarrays (Gene Chips)  461

21. Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology - Phage Display  471

22. Dowm-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology  493