PCR Technology : current innovations / edited by Thomas, Weissensteiner ... [et al.]. --2nd ed. -- CRC, 2004

Contents

1. Extraction and Amplification of Ancient DNA  5

2. Long PCR from Damaged DNA  11

3. Quantitative mRNA Analysis in Small Cell Samples by RT-PCR and Flow Cytometry  23

4.Microdissection of Single or Small Numbers of Cells for Analyses of DNA and RNA by PCR  29

5. Pre-PCR Processing Strategies  37

6. Novel PCR-Enhancing Compounds and Their Modes of Action  51

 7. Optimizing PCR with the Aid of Experimental Design  65

8. Economic Fluorescent Labeling of PCR Products for Microsatellite and Single-Stranded  Conformation Polymorphism (SSCP) Analysis  79

9. Universal Restriction Site-Free Cloning Method Using Chimeric Primers  87

10. High-Throughput Genotyping and the PCR  97

11. High-Throghput Genetic Analysis through Multiplexed PCR and Multicapillary Electrophoresis  111

12. The GOOD Assay: A Purification-Free Assay for Genotyping by MALDI  Mass Spectrometry  121

13. Primer Design for Larg-Scale Multiplex PCR and Arrayed Primer Extension (APEX)  131

14. Surface Plasmon Resonance Based Biosensor Technology for Real-Time Detection of PCR Products  141

15. Fluorescent Amplified Fragment Length Polymorphism (FAFLP) Genotyping  155

16. Global Analysis of DNA Allelic Variations (GADAV) by Spectific  163

17. Quantitative DNA-Methylation Analysis by the Bisulfite Conversion Method  175

18. Pyrosequencing Technology  187

19. Octamer-Primed Cycle Sequencing  197

20. In Situ Amplification of cDNA  205

21. Detection of a Single-Copy Sequence on Human Metaphase by Cyclic PRINS  215

22. Meaningful Quantification of mRNA Using Real-Time PCR  225

23. Absolute Quantification of Specific Nucleic Acids by RT-PCR Using a Nonlinear Mathematical Model for Data Analysis  235

24. Single-Molecule PCR- Basic Protocols and Applications  245

25. Gene Expression and Discovery: Application of a Genome-Wide Approach  259

26. The Detection of Differential Gene Expression Changes Using SAGE  267

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