HPLC of Peptides and Proteins : methods and protocols / edited by Marie-Isabel Aguilar. -- (Methods in Molecular Biology ; v.251). -- Humana Press, 2004

Contents

索书号: 58.17/M592/V.251

Contents:

1.HPLC of Peptides and Proteins: Basic Theory and Methodology  3

2.Reversed-Phase High-Performance Liquid Chromatography  9

3.Ion-Exchange Chromatography  23

4. High-Performance Hydrophobic Interaction Chromatography  45

5.Gel Filtration Chromatography  55

6.Hydrophilic Interaction Chromatography  75

7.Immobilized Metal Ion Affinity Chromatography of Proteins  89

8.Immunoaffinity Chromatography of Proteins  103

9.Liquid Chromatography-Mass Spectrometru and Tandem Mass Spectrometry of Peptides and Proteins  111

10.Capillary Separations  143

11.Micropreparative HPLC of Peptides and Proteins  165

12.Multidimensional HPLC Purification of Proteins  177

13.Analytical High-Performance Liquid Chromatography  183

14.Prep/Semiprep Separations of Peptides  191

15.Large-Scale Protein Chromatography  211

16.Proteolytic Peptide Mapping  227

17.Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation  245

18.Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry  263

19.HPLC in the Analysis of Peptide Metabolism  275

20.Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells  291

21.Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques  307

22.HPLC and Mass Spectrometry of Intrinsic Membrane Proteins  323

23.IgG Purification  341

24.DNA-Binding Proteins: LC-MS tp Identify Key Domains in RNA Polymerase-Promoter Interactions  35125.Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC  365

26.Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry  377

27. Proteome Analysis  387