Calcium signaling protocols /edited by David G. Lambert. --Humana Press, 2005. --(58.17/M592/v.312)

Contents

Contents:

1.Fluorescent Measurement of [Ca²⁺]: Basic Practical Considerations  3

2. Measurement of  [Ca²⁺] in Whole Cell Suspensions using Fura-2  37

3. Measurement of  [Ca²⁺] in Cell Suspensions Using Indo-1  47

4. Confocal Microscopy: Theory and Applications for Cellular Signaling  57

5. Single-Cell and Subcellular Measurement of Intracellular  Ca²⁺    Concention  87

6. Ratiometric Ca2+ Measurements Using the FlexStation Scanning Fluorometer

7. Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging

    Plate Reader  125

8. Measurement of Ca2+ Entry Using 45Ca2+ Channel Currents: Heterologous Expression Systems and Dissociated Brain Neurons  161

9. Measurement of [3H] PN200-110 and [125] Conotoxin MVIIA Binding  135

10.Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents: Heterologous Expression Systems and Dissociated Brain Neurons  161

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