Pharmacogenomics :methods and protocols /edited by Federico Innocenti. --Humana Press, 2005. --(58.17/M592/v.311)

Contents

Contents:

1. Pharmacogenomics: Historical Perspective and Current Status  3

2.Transfection Assays With Allele-Specific Constructs: Functional Analysis

    of UDP-Glucuronosyltransferase Variants  19

3.Snapshot of the Allele-Specific Variation in Human Gene Expression  31

4. Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo

    Microarrays  39

5. HaploChIP: An In Vivo Assay  49

6. Aspects Influencing Genotyping Methods Selection  63

7. Denaturing High-Performance Liquid Chromatography for Mutation Detection

    and Genotyping  73

8. Pyrosequencing of Clinically relevant Polymorphisms  97

9. Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation  115

10. Matrix-Assisted Laser Desorption/Lonization Time-of-Flight Mass

      Spectrometry  125

11. Fluorescence-Based Fragment Size Analysis  139

12. Single-Nucleotide Polymorphism Genotyping in DNA Pools  147

13. TaqMan Genotyping of Insertion/Deletion Polymorphisms  165

14. PharmGKB: The Pharmacogenetics and Pharmacogenomics Knowledge

       Base  179

15. Systems for the Management of Pharmacogenomic Information  193