DNA repair ptotocols :mammalian systems /edited by Daryl S. Henderson. --Humana Press, 2005. --(58.17/M592/v.314)

Contents

Contents:

1. Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by

    Replica Plating  1

2.Complementation Assays Adapted for DNA Repair-Deficient Keratinocyte  9

3. Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities  25

4. Evaluating the Delayed Effects of Cellular Exposure to lonizing Radiation  43

5. Inhibition of DNA Synthesis by lonizing Radiation: A Marker for an S-Phase

    Checkpoint  51

6. Analysis of Inhibition of DNA Replication in Irradiated Cells Using the

    SV40-Based in Vitro Assay of DNA Replication  61

7. Cytometric Assessment of Histone H2AX Phosphorylation:

     A Reporter of DNA Damage  73

8. Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in

    Studies of Apoptosis and Cell Proliferation (DNA Replication)  81

9. In Vitro Rejoining of Double-Strand Breaks in Genomic DNA  95

10. Detection of DNA Double-Strand Breaks and Chromosome Translocations

      Using Ligation-Mediated PCR and Inverse PCR  109

11. Plasmid-Based Assays for DNA End-Joining in Vitro  123

12. Use of Gene Targeting to Study Recombination in Mammalian Cell

       DNA Repair Mutants  133

13. Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage  155

14.Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA

      Damage and Repair in Mammalian Cells  183

15. Measuring the Formation and Repair of DNA Damge by Ligation-Mediated

       PCR  201

16. Immunochemical Detection of UV- Induced DNA Damage and Repair  215

17. A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet

      Photoproducts  229

18. Quantification of Photoproducts in Mammalian Cell DNA Using

       Radioimmunoassay  239

19. DNA Damage Quantitation by Aikaline Gel Electrophoresis  251

20. The Comet Assay: A Sensitive Genotoxicity Test for the Detection

       of DNA Damage and Repair  275

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