A Laboratory Guide to the Mammalian Embryo / edited by David K. Gardner ... [et.al.]. -- Oxford, 2004

索书号:59.115/L123a

Contents:

1.Follicle Development in Vitro  3

2.Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer  24

3.Culture of the Mammalian Preimplantation Embryo  41

4.Assessment of Preimplantation Embryo Development and Viability  62

5.Micromanipulation of Gametes  76

6.Micromanipulation: Biopsy  99

7.Analysis of Intracellular Ions in Embryos:pH and Calcium  125

8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity  139

9.Metabolic Pathway Activity  154

10.Confocal Imaging of Structural Molecules in Mammalian Gametes  165

11.Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis  184

12.Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos  193

13.Microscale RNA Isolation from Mammalian Embryos  217

14.Relative mRNA Transcript Abundance in Early Embryyos by Reverse Transcription-Polymerase Chain Reaction  225

15.Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR  237

16.Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells  247

17. Antisense Distruption of Gene Expression in the Preimplantation Embryo  258

18.Gene Subtration and Analysis  270

19.Analysis of Apoptosis in the Preimplantation Embryo  279

20.Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo  298

21.Human Embryonic Stem Cells  313

22.Spermatogonial Transplantation  334

23.Somatic Cell Nuclear Transfer  352

24.Cryobiology: Slow Freezing Vitrification of Embryos  375