Mobile genetic elements : protocols and genomic applications / edited by Wolfgang J. Miller. -- (Methods in molecular biology ; v. 260). -- Human Press, 2004. -- (58.17/M592/v.260)

Contents:

1. Mobile Genetic Elements as Natural Tools for Genome Evolution   1

2. Deterction of Transposable Elements in Drosophila Salivary Gland Polytene Chromosomes by In Situ Hybridization  21

3. Fluorescent in situ Hybrization With Transposable Element Probes to Mitotic chromosomal-heterochromatin of Drosophila  29

4. Southern Blot Analysis of Individual Drosophila Flies  41

5. Computational Analysis of Transposable Element Sequences  59

6. Biochemical Analysis of Long Terminal Repeat Retrotransposons  72

7. Tn5 as a Molecular Genetics Tool: In Vitro Transposition and the Coupling of in Vitro Technologies With in Vivo Transposition  83

8. Gene Targeting in Drosophila  97

9. TE-Based Mutagenesis Systems in Plants: A Gene Family Approach  115

10. The Use of Double-Stranded RNA to Knock Down specific Gene Activity  129

11. The Application of LTR Retrotransposons as Molecular markers in Plants  145

12. MITE Display  175

13. Retroposon Mapping in Molecular Systematics  189

14. Transformation systems in Insects  227

15. Transposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates: A Contemporary Review of Experimental Strategies  255