Biopolymer methods in tissue engineering / edited by Anthony P. Hollander. -- (Methods in molecular biology ; v. 238). -- Humana Press, 2004. -- (58.17/M592/v.238)

Contents:

1. Processing of Resorbable Poly- α-Hydroxy Acids for Use as Tissue-Engineering Scaffolds  1

2. Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing  11

3. Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering  25

4. Synthesis and characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering  41

5. Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2  49

6. Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering applications  65

7. Alginates in Tissue Engineering  77

8. Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering  87

9. Modification of Materials With Bioactive Peptides  113

10. Isolation and Ostrogenic differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineering  123

11. Cell Seeding of Polymer Scaffolds  131

12. Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds  147

13. Bioreactor Culture Techniques for Cartilage-Tissue Engineering  159

14. Microscopic Methods for the analysis of Engineered Tissues  171

15. Transmission electron Microscopy of Tissue-Polymer Constructs  197

16. Application of Microscopic Methods for the Detection of Cell Attachment to Polymers  207

17. Biochemical Methods for the Analysis of Tissue-Engineered Cartilage  217

18. Real-Time Quantitative RT-PCR Assays  231

19. Mechanical Testing of Cell-Material Constructs: A Review  239