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SH2 domains : functional modules and evolving tools in biology / edited by Teresa Carlomagno, Maja Köhn. -- New York, NY : Springer, 2023. – (58.17/M592/v.2705) |
Contents
PART I STRUCTURE AND DYNAMICS OF SH2 DOMAINS
1 Methods for Structure Determination of SH2 Domain-Phosphopeptide Complexes by NMR
2 NMR Methods to Study the Dynamics of SH2 Domain-Phosphopeptide Complexes
3 Crystal Structure Analysis of SH2 Domains in Complex with Phosphotyrosine Peptides
4 Revealing Allostery in PTPN11 SH2 Domains from MD Simulations
5 Structure Determination of SH2-Phosphopeptide Complexes by X-Ray Crystallography: The Example of pl20RasGAP
PART II BIOPHYSICS AND BIOINFORMATICS OF SH2 DOMAIN--PHOSPHOPEPTIDE BINDING
6 Fluorescence Anisotropy and Polarization in the Characterization of Biomolecular Association Processes and Their Application to Study SH2 Domain Binding Affinity
7 Computational Evaluation of Peptide-Protein Binding Affinities: Application of Potential of Mean Force Calculations to SH2 Domains
8 NMR Relaxation Dispersion Experiments to Study Phosphopeptide Recognition by SH2 Domains: The Grb2-SH2-Phosphopeptide Encounter Complex
9 Using Linear Motif Database Resources to Identify SH2 Domain Binders
10 Using Surface Plasmon Resonance to Study SH2 Domain-Peptide Interactions
PART III SMALL-MOLECULE BINDERS AND INHIBITORS OF SH2 DOMAINS
11 Inhibitor Library Screening of SH2 Domains Through Denaturation-Based Assays
12 Dissecting Selectivity Determinants of Small-Molecule Inhibitors of SH2 Domains Via Fluorescence Polarization Assays
13 Lipid Binding of SH2 Domains
14 Exploring the Binding Interaction Between Phosphotyrosine Peptides and SH2 Domains by Proximal Crosslinking
15 Synthesis and Biochemical Evaluation of Monocarboxylic GRB2 SH2 Domain Inhibitors
PART IV ENGINEERING SH2 DOMAINS
16 Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites
17 Engineering SH2 Domains with Tailored Specificities and Affinities
PART V SH2-DOMAIN CONTAINING PROTEINS AND REGULATION OF ACTIVITY
18 Measuring Protein Tyrosine Phosphatase Activity Dependent on SH2 Domain-Mediated Regulation
19 Peptides as Baits for the Coprecipitation of SH2 Domain-Containing Proteins
20 Biomolecular Condensation of SH2 Domain-Containing Proteins on Membranes
Index
