Cancer cell culture : methods and protocols / edited by Dania Movia, Adriele Prina-Mello. -- New York, NY : Humana Press, 2023. – (58.17/M592/v.2645)

Contents

Preface
Contributors.
PART I  OVERVIEW OF THE EXISTING METHODS FOR CANCER CELL CULTURE
1  Cancer Cell Culture: The Basics and Two-Dimensional Cultures
2  Cell Cultures at the Air-Liquid Interface and Their Application in Cancer Research
3  Three-Dimensional Spheroids for Cancer Research
4  Disease Animal Models for Cancer Research
PART II  SPECIFIC METHODS FOR CANCER CELL CULTURE: STEP-BY-STEP METHODOLOGIES
5  Generation of Radioresistant Prostate Cancer Cells
6  Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocarcinoma
7  Developing an In Vitro Isogenic Model of Chemotherapy-Resistant Lung Cancer
8  Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environment
9  A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air-Liquid Interface
10  Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cancer Patients
11  Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research
12  A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture
13  A Step-by-Step Methodological Guide for Developing Zonal Multicellular Scaffold-Based Pancreatic Cancer Models
14  Measuring Immune Cell Movement Toward the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay
15  A Method for the In Vitro Cytotoxicity Assessment of Anti-cancer Compounds and Materials Using High Content Screening Analysis
16  Testing the Effects of Magnetic Hyperthermia in 2D Cell Culture
17  Cell Viability Assay with 3D Prostate Tumor Spheroids
18  Analysis of Cancer Cell Line Secretomes: A Complementary Source of Disease-Specific Protein Biomarkers
Correction to: Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research
Index