Cell-secreted vesicles : methods and protocols / edited by Seppo Vainio. -- New York, NY : Humana Press, 2023. – (58.17/M592/v.2668)

Contents

Preface
Contributors
PART I  PURIFICATION AND ANALYSIS OF SINGLE OR MULTIPLE EVs
1  Plasmon-Enhanced Characterization of Single Extracellular Vesicles
2  Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles
3  In Vivo Analysis of Heterogeneous Extracellular Vesicles Using a Red-Shifted Bioluminescence Resonance Energy Transfer Reporter Protein
4  Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabeling Electron Microscopy
5  Extracellular Vesicle Isolation by a Tangential-Flow Filtration-Based Large-Scale Purification Method
6  Metabolomics Analysis of Urinary Extracellular Vesicles by Nuclear Magnetic Resonance and Liquid Chromatography-Mass Spectrometry
7  Methodologies for Scalable Production of High-Quality Purified Small Extracellular Vesicles from Conditioned Medium
8  Automated On-Line Isolation and Fractionation Method for Subpopulations of Extracellular Vesicles
PART II  TARGETING CELL BEHAVIOR FUNCTIONS OF EVs
 9  A Novel Assay for Investigating the Role of Exosomes in Tumor Cell-Endothelial Cell Crosstalk
10  Nanoparticle (NP) Loading by Direct Incubation with Extracellular Vesicles-Secretor Cells: NP Encapsulation and Exosome Characterization
11  Two Complementary Strategies to Quantitate Extracellular Vesicle Uptake Using Bioluminescence and Non-Lipidic Dyes
12  Integrin-Mediated Exosomal Homing to Organs
13  Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research
14  Quantitative Analysis of Extracellular Vesicle Release Using Artificial MicroRNAs
PART III  SYSTEMS AND MODELS TO STUDY EVs
15  Purification of Bacterial-Enriched Extracellular Vesicle Samples from Feces by Density Gradient Ultracentrifugation
16  Isolation and Characterization of Extracellular Vesicles from Lymphocytes
17  Biogenesis of Mesoporous Silica Nanoparticles Enclosed in Extracellular Vesicles by Mouse Renal Adenocarcinoma Cells
18  Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications
19  Cilia-Derived Extracellular Vesicles in Caenorhabditis Elegant. In Vivo Imaging and Quantification of Extracellular Vesicle Release and Capture
20  Exosome-Based COVID-19 Vaccine
Index