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Exocytosis : from molecules to cells / edited by Arun Anantharam, Jefferson Knight. -- Bristol, UK : IOP Publishing, 2022. – (58.1574/E96) |
Contents
1 Introduction
1.1 Purpose and scope of this e-book References and recommended reading
Part I Components of exocytotic pathways
2 Biogenesis of secretory granules
2.1 Introduction
2.2 Secretory protein sorting beginning in the TGN
2.3 The roles of lipids in the early events of SG formation
2.4 Scission of nascent IGs from the TGN
2.5 Granule maturation a multi-step process
2.6 Granule subpopulations in regulated secretory cells
2.7 Exploring SG biogenesis where we are and going forward
Acknowledgments
References
3 Exocytotic SNARE complex assembly, disassembly, and regulation
3.1 Introduction to exocytotic SNARE proteins and Fusion events at the neuronal plasma membrane
3.2 Mechanisms of SNARE complex assembly
3.3 Negative regulation of the SNARE complex assembly by tomosyn-I and amisyn
3.4 Disassembly of the SNARE complex and reuse of the SNARE proteins
3.5 SNARE-mediated membrane fusion beyond 2022: functional diversity of non-canonical SNARE proteins
Acknowledgements
References and further reading
4 Secretory granule motions adjacent to the plasma membrane and granule membrane protein mobility: implications for exocytosis
4.1 Summary
Acknowledgements
References
5 Fusion pore stability and dynamics
5.1 Techniques for studying fusion pores
5.2 Functional considerations
5.3 The energy landscape of membrane fusion
5.4 Fusion pore states
5.5 Conclusions
References
6 Mechanical regulation of exocytosis and endocytosis
6.1 Introduction
6.2 Membrane tension and membrane homeostasis
6.3 Coupling of exo- and endocytosis
6.4 Regulation of exocytosis by membrane tension
6.5 Regulation of endocytosis by membrane tension
6.6 Outlook
References
7 A dynamin perspective on endocytosis and coupling to exocytosis
7.1 Introduction
7.2 Dynamin's role in endocytosis
7.3 Mechanochemical properties of dynamin leading to membrane fission
7.4 Coupling endocytosis and exocytosis
7.5 Summary
References
Part II Biophysical insights from synthetic and reconstituted systems
8 Understanding the molecular mechanism of fusion pores through reconstruction
8.1 Introduction
8.2 SNARE-mediated membrane fusion
8.3 The mystery of fusion pores
8.4 Interrogation of fusion pores using liposomes
8.5 Isolation of nascent fusion pores using nanodiscs
8.6 Dissection of pore properties using electric recording
8.7 Reconstitution of expanded fusion pores
8.8 Future directions
Acknowledgments
References
9 Pore-spanning membranes: a tool for studying neuronal fusion
9.1 Introduction
9.2 hi vitro fusion assays
9.3 Setting up a single-vesicle assay based on pore-spanning membranes
9.4 Single-vesicle fusion assay lipid mixing
9.5 Impact of synaptotagmin-1 on fusion efficiency and kinetics
9.6 Single-vesicle fusion assay content release
9.7 Conclusions
Acknowledgements
References
10 Effects of anesthetics on membrane fusion and exocytosis
10.1 Introduction
10.2 Neurotransmission
10.3 Current hypotheses of general anesthesia
10.4 Membrane-membrane interactions
10.5 Summary and conclusions
Acknowledgments
References
Part III Physiological systems of exocytosis
11 Exocytosis in yeast: major players and mechanisms
11.1 Membrane trafficking in the yeast physiology
11.2 The role and regulation of the exocyst in vesicle tethering during exocytosis
11.3 The role and regulation of SNAREs in vesicle tethering and fusion during exocytosis
11.4 Small Rab GTPases
11.5 The cytoskeleton and small Rho GTPases: roles in exocytosis
11.6 Outstanding questions
References
12 Stimulus-secretion coupling in the adrenal medulla
12.1 Anatomy and innervation
12.2 Cholinergic transmission in the medulla: the role of nicotinic receptor
12.3 Cholinergic transmission in the medulla: the role of muscarinic receptors
12.4 Peptidergic transmission in the medulla: the role of PACAP
12.5 Spontaneous versus evoked activity in the medulla
12.6 Activity-dependent remodeling of the adrenal medulla
12.7 Concluding perspectives
Acknowledgements
References
13 Synaptic vesicle dynamics at the calyx of Held and other central synapses
13.1 Regulation of release probability at the calyx of Held
13.2 Regulation of release probability at other synapses
13.3 Synaptic vesicle pools at the calyx of Held
13.4 Vesicle pools at other synapses
13.5 Visualization of synaptic vesicle dynamics at the calyx of Held and other synapses
13.6 Physiological relevance of vesicle pools at the calyx of Held and other central synapses
13.7 Summary
Acknowledgements
References
14 Mechanisms of exocytosis in mammalian fertilization
14.1 Overview of fertilization
14.2 Triggers of acrosomal exocytosis in mammalian sperm
14.3 Early stages of the AR: Rabs, Ca2+, and cAMP
14.4 Other regulatory proteins that control Ca2+-induced acrosomal exocytosis: complexins, synaptotagmins, and Munc proteins
14.5 Mammalian oocytes undergo constitutive exocytosis prior to oocyte maturation
14.6 Release of Ca2+ from intracellular stores in the egg is the trigger for cortical granule exocytosis after fertilization
14.7 SNARE proteins mediate constitutive exocytosis and CGE
Acknowledgments
References
15 Molecular regulation of multivesicular endosome fusion and exosome secretion
15.1 Introduction
15.2 Exosomes in physiology
15.3 Exosomes in disease slates
15.4 The diversity of multivesicular endosomes, intraluminal vesicles and exosomes
15.5 Exosome analysis methods
15.6 Mechanisms by which MVE secretion releases exosomes
15.7 Conclusions
Acknowledgements
References
