Carbohydrate-protein interactions : methods and protocols / edited by D. Wade Abbott, Wesley F. Zandberg. -- Second edition -- New York, NY : Humana Press, 2023. – (58.17/M592/v.2657)

Contents

Preface
Contributors
PART I  MEASUREMENT OF CARBOHYDRATE MODIFICATION
1  A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases
2  Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay
3  Analyzing Activities of Lyric Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry
4  Carbohydrate Depolymerization by Intricate Cellulosomal Systems
5  Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins
PART II  QUANTITATION OF CARBOHYDRATE BINDING
6  Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions
7  Quantifying CBM-Carbohydrate Interactions Using Microscale Thermophoresis
8  Qualitative and Quantitative Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy
9  Isothermal Titration Calorimetry for Quantification of Protein-Carbohydrate Interactions
10  Surface Plasmon Resonance Analysis for Quantifying Protein-Carbohydrate Interactions
11  Bioinspired Polymer Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP
12  CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry
13  Flow Cytometry-Based Detection of Siglec Ligands
PART III  PURIFICATION AND VISUALIZATION OF CARBOHYDRATES
14  Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material
15  Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy
16  Separation and Visualization of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis
17  A Rapid Protocol for Preparing 8-Aminopyrene-l,3,6-Trisulfonate-Labeled Glycans for Capillary Electrophoresis-Based Enzyme Assays
18  Visualization of Carbohydrate Uptake Using Fluorescent Polysaccharides ..
PART IV  COMPUTATIONAL METHODS FOR STUDYING PROTEIN- CARBOHYDRATE INTERACTIONS
19  Long-Read Metagenomics and CAZyme Discovery
20  Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics
21  Isolation and Preparation of Extracellular Proteins from Lignocellulose-Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry
Index