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Plant genome engineering : methods and protocols / edited by Bing Yang, Wendy Harwood, Qiudeng Que. -- New York : Humana Press, 2023. – (58.17/M592/v.2653) |
Contents
Preface
Contributors
PART I GENOME ENGINEERING SYSTEMS
1 PAM-Less CRISPR-SpRY Genome Editing in Plants
2 Type I-D CRISPR System-Mediated Genome Editing in Plants
3 CRISPR/LbCas12a-Mediated Genome Editing in Soybean
4 Base Editing in Poplar Through an Agrobacterium-Mediated Transformation Method
5 Genetic Engineering of Potato (Solanum tuberosum) Chloroplasts Using the Small Synthetic Plastome "Mini-Synplastome".
PART II DESIGN AND VALIDATION TOOLS
6 Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout Using CRISPR-BETS
7 Construction of CRISPR/Cas9 Multiplex Genome Editing System in Rice
8 Use of Fluorescent Protein Reporters for Assessing and Detecting Genome Editing Reagents and Transgene Expression in Plants
9 Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies
PART III TRANSGENE-FREE DELIVERY OF MACHINERY
10 Delivery of CKISPR-Casl2a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line
11 Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector
12 Ribonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.)
13 Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum vulgare L.) Leaves
14 Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide
PART IV GENERATION AND ANALYSIS OF ENGINEERED LINES
15 Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method
16 Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems
17 Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.)
18 A CRISPR/Cas9 Protocol for Target Gene Editing in Barley
19 Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration
20 Stepwise Optimization of Real-Time RT-PCR Analysis
21 CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding
PART V GENOME ENGINEERING FOR CROP IMPROVEMENT
22 Recent Advances in Engineering of In Vivo Haploid Induction Systems
Index
