Spermatogonial stem cells : methods and protocols / edited by Jon M. Oatley, Brian P. Hermann. -- New York : Humana Press, 2023. – (58.17/M592/v.2656)

Contents

Preface
Contributors
1  Introduction: The Why's and How's for Studying Spermatogenesis and Spermatogonial Stem Cells
2  Optimization of Testicular Fixation-Embedding Techniques for Improved Evaluation of Mammalian Spermatogonial Morphology and Function
3  Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes
4  Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia
5  Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell--Part 1: Epigenomics Assays
6  Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell: Part 2--Computational Analysis of Epigenomics Data
7  Primary Cultures of Spermatogonia and Testis Cells
8  Reconstitution of Human Prospermatogonial Development from Human-Induced Pluripotent Stem Cells
9  RNA Interference as a Method of Gene Knockdown in Cultured Spermatogonia
10  Isolation of Undifferentiated Spermatogonia from Adult and Developing Mouse Testes
11  Spermatogonial Transplantation
12  Viral Transduction of Mammalian Spermatogonial Stem Cells
13  Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis
14  Models and Methods for Evaluating Regeneration of Spermatogenesis After Cytotoxic Treatments
15  Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches
16  Lineage Tracing of Spermatogonial Stem Cells Within the Male Germline
17  Perspectives: Approaches for Studying Livestock Spermatogonia
18  Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells
Index