Cell polarity signaling : methods and protocols / edited by Chenbei Chang and Jianbo Wang. -- New York : Humana Press, 2022. – (58.17/M592/v.2438)

Contents

Preface
Contributors
1  Use of Fluorescence Recovery After Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins
2  FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs
3  Live-Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics
4  Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates
5  Biochemical Assays to Detect Activation of Small
6  Analysis of Planar Cell Polarity Complexes by Proximity Biotinylation in Xenopus Embryos
7  Regulation of Cell Polarity by Posttranslational Protein Palmitoylation
8  Enrichment and Detection of Wnt Proteins from Cell Culture Media
9  Using Live Imaging to Examine Early Cardiac Development in Zebrafish
10  Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm
11  In Ovo Gain- and Loss-of-Function Approaches to Study Gut Morphogenesis
12  Avian Embryos as a Model to Study Vascular Development
13  BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice
14  Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics
15  Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embryo
16  Methods to Investigate Cell Polarity of Inner Ear
17  Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice
18  In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance
19  Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance
20  Dissection, Fixation, and Immunostaining of the Drosophila Midgut
21  In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands
22  Imaging Epidermal Cell Rearrangement in the C. elegans Embryo
23  Methods for the Study of Apical Constriction During Ascidian Gastrulation
24  Assays for Apical Constriction Using the Xenopus Model
25  The Use of Three-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation
26  Studying Cell Polarity Dynamics During Cancer Initiation Using Inducible 3D Organotypic Cultures
27  Under-Agarose Chemotaxis and Migration Assays for Dictyostelium
28  Mapping Asymmetry in Collective Cell Migration: Lessons from Border Cells in Drosophila Oogenesis
29  A Toolbox to Study Tissue Mechanics In Vivo and Ex Vivo
30  Quantitative Analysis of Directional Neural Crest Cell Migration
Index