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Environmental responses in plants : methods and protocols / edited by Paula Duque, Dora Szakonyi. -- Second edition. -- New York, NY : Humana Press, 2022. – (58.17/M592/v.2494)

Contents

Preface
Contributors
PART I  TROPISMS AND OTHER DEVELOPMENTAL RESPONSES
1  Analysis of Plant Root Gravitropism
2  Hydrotropism: Analysis of the Root Response to a Moisture Gradient
3  Evaluating Root Mechanosensing Response in Rice
4  Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis
5  Shoot Branching Phenotyping in Arabidopsis and Tomato
6  A Ribo-Seq Method to Study Genome-Wide Translational Regulation in Plants
PART II  PHOTOPERIODISM AND CIRCADIAN RYTHMS
7  Assessing Flowering Time Under Different Photoperiods
8  Analysis of Phytochrome-Dependent Seed Germination in Arabidopsis
9  Rhythmic Leaf and Cotyledon Movement Analysis
10  Assessing Plant Pigment Regulation in Circadian Experiments
11  Chemical Perturbation of Chloroplast Ca2+ Dynamics in Arabidopsis thaliana Suspension Cell Cultures and Seedlings
PART III  ABIOTIC STRESS RESPONSES
12  Screening for Abiotic Stress Response in Rice
13  Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion
14  Assessing Postgermination Development in Arabidopsis thaliana Under Abiotic Stress
15  Assessing Abscisic Acid-Mediated Changes in Stomata] Aperture Through High-Quality Leaf Impressions
16  Evaluation of the Anti-transpirant Activity of ABA Receptor Agonists in Monocot and Eudicot Plants
17  Quantifying Phytohormones in Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset
18  Optical Imaging Resources for Crop Phenotyping and Stress Detection
PART IV  PLANT INTERACTIONS WITH OTHER ORGANISMS
19  Analysis of Innate Immune Responses Against Pathogenic Bacteria in Arabidopsis, Tomato, and Barley
20  Performing Infection Assays of Sporisorium reilianum f. sp. Zeae in Maize
21  A Method to Measure the Damage Caused by Cell-Sucking Herbivores
22  RNA Isolation from Nematode-Induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection
23  High-Throughput Profiling of Root-Associated Microbial Communities
24  Analysis of Immunity-Related Oxidative Bursts by a Luminol-Based Assay
Index