Microfluidics for cellular applications / edited by Gerardo Perozziello, Ulrich Kruhne, Paola Lucinani. -- Amsterdam, Netherlands : Elsevier, 2023. – (58.17111/M626)

Contents

List of contributors
CHAPTER 1 Introduction
CHAPTER 2 The cell: cell microenvironment and cell handling
2.1 What is a cell?
2.2 Different cell types
2.3 Biomarkers
2.4 Cell culture basics
2.5 Typical workflow for subculturing adherent cells
2.6 Usage of biomarkers in cell culture
2.7 Cellular staining
2.8 Instruments to analyze/process stained cells
References
CHAPTER 3 Microfluidic devices and their applicability to cell studies
3.1 Introduction
3.2 Examples of microfluidic platforms applied to different cell applications.
3.3 Conclusion
References
CHAPTER 4 Materials
4.1 Overview
4.2 Material classification for microfluidic platforms
4.3 Biocompatibility
4.4 Requirements in relation to the cell-surface interface
4.5 Material requirements in relation to (optical, electrochemical) sensor interface
4.6 Fluidic compatibility (liquids and gases)
4.7 Conclusion
References
CHAPTER 5 Surface properties and treatments
5.1 Surface properties
5.2 Surface modification
5.3 Biological surface functionalization
5.4 Surface topographical modifications
5.5 Combined plasma and silanization method to immobilize biomolecules on microfluidic surfaces
5.6 Conclusion
References
CHAPTER 6 Fabrication technologies
6.1 Standard lithographic process
6.2 Resists
6.3 Optical lithography
6.4 Electron beam lithography
6.5 Etching processes
6.6 Cost-effective fabrication technologies
6.7 Fabrication of microfluidic devices
References
CHAPTER 7 Handling and control setups for microfluidic devices
7.1 Generalities on control systems
7.2 Design and implementation of control systems
7.3 Feedforward and feedback control
7.4 Amplifiers
7.5 Control systems for microfluidic devices
7.6 PID basics
7.7 Feedback control system of a cell incubator: design and implementation
7.8 Microfluidic device interface
7.9 Microscopy techniques
7.10 Spectroscopy techniques
7.11 Liquid driving methods
References
CHAPTER 8 Commercial microfluidic devices and their cost analysis
8.1 Introduction
8.2 Key facts about the commercial microfluidic market
8.3 Typical product cycle from the development phase into production
8.4 The strategy
8.5 Cost drivers in microfluidic devices
8.6 Summary
References
CHAPTER 9 Microfluidics for nanopharmaceutical and medical applications
9.1 Introduction
9.2 Nanoparticulate drug delivery systems for pharmaceutical
9.3 From microfluidic 3D cell culture to human-on-a-chip
9.4 Conclusion
Acknowledgement
References
Index