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Lipidomics : methods and protocols / edited by Sanjoy K. Bhattacharya. -- Second edition -- New York, NY : Humana Press, 2023. – (58.17/M592/v.2625)

Contents

Preface
Contributors
1  Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis
2  Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation
3  Multi-macromolecular Extraction from Endosymbiotic Anthozoans
4  High-Resolution Liquid Chromatography-Mass Spectrometry for Lipidomics
5  Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry
6  Protocol and Methods Applicable to Retinal Vascular Diseases
7  Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics
8  Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics
9  Lipidomics Analysis with Triple Quadrupole Mass Spectrometry
10  High-Performance Chromatographic Separation of Cerebrosides
11  Quantification of Endocannabinoids from Biological Samples Using Liquid Chromatography-Tandem Mass Spectrometry
12  Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains
13  Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry
14  Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry
15  A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipid Droplets
16  Capillary Electrophoresis Plasma Fractionation for Lipids Analysis
17  Combined Use of MALDI-TOF Mass Spectrometry and 31p NMR Spectroscopy for the Analysis of (Phospho)Lipids
18  Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles (OMVs) of Pseudomonas Aeruginosa
19  Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells
20  Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate
21  Lipid Extraction and Sample Preservation Techniques for Stable Isotope Analysis and Ecological Assays
22  Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry
23  Untargeted Analysis of Lipids Containing Very Long Chain Fatty Acids in Retina and Retinal Tight Junctions
24  Analysis of Lipid Contents in Human Trabecular Meshwork Cells by Multiple Reaction Monitoring (MRM) Profiling Lipidomics
25  Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples
26  Downloading and Analysis of Metabolomic and Lipidomic Data from Metabolomics Workbench Using MetaboAnalyst 5.0
27  Accurate Analysis of Lipid Building Blocks Using the Tool LipidOne
28  Image-Based Longitudinal Characterization of Corneal Wound to Understand the Role of Sphingosine-l-Phosphate
29  Characterization of Trabecular Meshwork Mechanical Property Modulation After Application of Lipids
30  C-Laurdan: Membrane Order Visualization of HEK293t Cells by Confocal Microscopy
31  Sonication-Based Basic Protocol for Liposome Synthesis
Index