Pyroptosis : methods and protocols / edited by Susan L. Fink. -- New York, NY : Humana Press, 2023. – (58.17/M592/v.2641)

Contents

Preface
Contributors
1  Molecular Mechanisms of Pyroptosis
2  Assessment of NLRP3 Inflammasome Activation and NLRP3-NEK7 Complex Assembly
3  Inducing Pyroptosis with FlaTox, RodTox, or NeedleTox
4  Inducing Pyroptosis Via the Pyrin Inflammasome
5  Caspase-4 Activation and Recruitment to Intracellular Gram-Negative Bacteria
6  CARD8 Inflammasome Activation by HIV-1 Protease
7  Pyroptosis Markers in Human Primary Specimens: Quantification of Intracellular ASC Specks by Imaging Flow Cytometry and Extracellular Oxidized Mitochondrial by ELISA
8  Identifying Inflammasome Activation in Human Tissues
9  Analyzing Caspase-8-Dependent GSDMD Cleavage in Response to Yersinia Infection
10  Biochemical Methods for Assessing Gasdermin D Inactivation in Macrophages
11  Detection of Gasdermin C-Mediated Cancer Cell Pyroptosis
12  Inducing Pyroptosis via Gasdermin B and Gasdermin E Cleavage
13  Assessing Interleukin- lβ Activation During Pyroptosis
14  Monitoring Calcium Fluxes and Lysosome Exocytosis During Pyroptosis..
15  Assessing Cytoskeletal Destruction During Pyroptosis
16  Assessment of HMGB1 Release During Experimentally Induced Pyroptosis
17  Time-Lapse Imaging of Inflammasome-Dependent Cell Death and Extrusion in Enteroid-Derived Intestinal Epithelial Monolayers
Index