DNA manipulation and analysis / edited by Garry Scarlett. -- New York, NY : Humana Press, 2023. – (58.17/M592/v.2633)

Contents

Preface
Contributors
1  Classical Recombinant DNA Cloning
2  A Sequence- and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector
3  Molecular Cloning Using In Vivo DNA Assembly
4  Assembling Multiple Fragments: The Gibson Assembly
5  TA Cloning Approaches to Cloning DNA with Damaged Ends DNA
6  PCR-Based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis
7  Random Mutagenesis by PCR
8  In Vitro Site Directed Mutagenesis
9  Xenopus Transgenesis Using the pGateway System
10  CRISPR/Cas9 Gene Disruption Studies in F0 Xenopus Tadpoles: Understanding Development and Disease in the Frog
11  A CRISPR/Cas-Based Method for Precise DNA Integration in Xenopus laevis Oocytes Followed by Intracytoplasmic Sperm Injection (ICSI) Fertilization
12  A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets
13  Generation of Functional-RNA Arrays by In Vitro Transcription and In Situ RNA Capture for the Detection of RNA-RNA Interactions
14  Chemical Synthesis of Oligonucelotide Sequences: Phosphoramidite Chemistry
15  Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products
16  Nanopore Sequencing for Mixed Samples
17  Ethics, Legality, and Safety for Geneticists
Index