Molecular biology and biotechnology / edited by Ralph Rapley, University of Hertfordshire, UK. -- 7th edition. -- London : Royal Society of Chemistry, [2021]. – (58.17/M718c/7th ed.)

Contents

1 Basic Molecular Biology Techniques
     1.1 Introduction
     1.2 Structure of Nucleic Acids
     1.3 Isolation and Separation of Nucleic Acids
     1.4 Basic Mapping and Analysis of DNA Fragments
     1.5 Gene Probes and Artificial DNA Synthesis
     1.6 The Polymerase Chain Reaction (PCR)
     1.7 Quantitative or Real-time PCR (qPCR)
     1.8 DNA Sequencing
     1.9 Microarrays
     1.10 Conclusion
     References
2 Genes and Genomes
     2.1 Introduction
     2.2 Recombinant DNA Technologies
     2.3 Genome Analysis
     2.4 Genome Projects Background
     2.5 Gene Discovery and Localization
     2.6 Conclusion
     References
3 Protein Expression and Production
     3.1 Introduction
     3.2 Host-related Issues
     3.3 Vectors
     3.4 Expression Problems
     3.5 Fusion Proteins
     3.6 Cell-free Systems
     3.7 High-throughput Approaches
     3.8 Other Hosts
     3.9 Conclusion
     References
4 Proteins and Proteomics
     4.1 Introduction
     4.2 Protein Chemistry
     4.3 Proteomics Workflow: Sample Preparation
     4.4 Electrophoretic Methods
     4.5 2D Gel Electrophoresis
     4.6 Protein Identification: Mass Spectrometry
     4.7 Protein Identification: Bioinformatics
     4.8 Expression Proteomics
     4.9 Practical Applications
     4.10 Conclusion
     Bioinformatics Sources Cited in the Text
     References
5 Transgenesis                         1
     5.1 Introduction
     5.2 Transgenesis by DNA Pronuclear Injection
     5.3 Gene Targeting by Homologous Recombination in Embryonic Stem Cells
     5.4 Conditional Gene Targeting
     5.5 Tetracycline-inducible Gene Switch System
     5.6 Genome Editing by Engineered Endonucleases
     5.7 Phenotypic Analysis of Genetically Modified Mice
     5.8 Ethical and Animal Welfare Considerations
     5.9 Conclusion
     Acknowledgements
     References
6 The Biotechnology and Molecular Biology of Yeast
     6.1 Introduction
     6.2 Production of Heterologous Proteins by Yeast
     6.3 Sophisticated Recombinant Cloning Techniques for the Large-scale Manipulation of DNA
     6.4 Development and Application of Post-genomic Analysis Techniques
     6.5 Rational Design of Yeast Biology
     6.6 Future Prospects
     References
7 Antibody Engineering and Immunotherapeutics
     7.1 Introduction
     7.2 Antibody Structure and Function
     7.3 Hybridoma Technology
     7.4 Therapeutic Antibodies
     7.5 Molecular Engineering of Antibodies
     7.6 Surface Display Technology
     7.7 Production of Antibody Fragments
     7.8 Bispecific Antibodies
     7.9 Molecular Redirection of Immune Cells
     7.10 Single-cell Technology and Transgenics
     7.11 Conclusion
     References
8 Human and Animal Cell Culture
     8.1 Introduction
     8.2 Understanding and Standardising In Vitro Culture Systems
     8.3 Quality Assurance
     8.4 Documentation
     8.5 Safety
     8.6 Legal, Ethical and Regulatory Compliance
     8.7 Education and Training
     8.8 Conclusion
     References
9 Genome Editing
     9.1 Background
     9.2 Targeted Mutagenesis Using ZFNs
     9.3 Genome Editing with TALENs
     9.4 The CRISPR/Cas System
     9.5 Case Study: CRISPR/Cas9 Gene Editing in Crop Plants
     9.6 Ethical and Regulatory Issues Related to Genome Editing
     References
10 Genome Sequencing
     10.1 Introduction
     10.2 A Brief History of DNA Sequencing
     10.3 Preparing for Next-generation Sequencing
     10.4 Data Analysis
     References
11 Introduction to Bioinformatics
     11.1 Introduction
     11.2 Primary and Secondary Databases
     11.3 Bioinformatics Data Formats
     11.4 NCBI BLAST and PubMed Resources
     11.5 Protein Databases
     11.6 Specialised Disease-related Databases
     11.7 Secondary and Specialised Databases
     11.8 Laboratory Bioinformatics
     11.9 Concluding Remarks
     References
12 Nanotechnology in Medicine
     12.1 Introduction
     12.2 Nanomaterials
     12.3 Clinically Approved NPs
     12.4 Future Prospects
     References
13 Biosensors
     13.1 Introduction
     13.2 The Biological Reaction
     13.3 Theory
     13.4 Electrochemical Methods
     13.5 Piezoelectric Biosensors
     13.6 Optical Biosensors
     13.7 Whole-cell Biosensors
     13.8 Receptor-based Sensors
     13.9 Conclusion
     References
14 Modelling and Simulation of Proteins
     14.1 Introduction
     14.2 Prediction of Protein Structure
     14.3 Molecular Dynamics Simulation
     14.4 Conclusion
     References
15 Agricultural Biotechnology
     15.1 Introduction
     15.2 Today's Agricultural Biotechnologies
     15.3 Biotic Stresses
     15.4 Tolerance to Abiotic Stresses
     15.5 Improved Yield and Nutrition Using Genetic Modification
     15.6 Potential to Improve Human Health via the Biofortification and Nutritional Enhancement of Food Crops
     15.7 Phytoremediation Using Agricultural Biotechnology
     15.8 Agricultural Biotechnology and Biofuel Development
     15.9 Consumer Acceptance of Plant Biotechnology
     15.10 Conclusion
     References
16 Vaccine Design Strategies: Pathogens to Genomes
     16.1 Introduction
     16.2 Immunological Considerations in Vaccine Design
     16.3 Vaccine Design Strategies
     16.4 Reverse Vaccinology: Genome-based Vaccine Design
     16.5 Recombinant Vector Vaccines
     16.6 Nucleic Acid-based Vaccines
     16.7 Synthetic Peptide Vaccines
     16.8 Vaccine Adjuvants and Delivery Systems
     16.9 Concluding Remarks
     References
17 Intellectual Property and Biotechnology Patents
     17.1 Background to Patents
     17.2 CRISPR Patents
     17.3 Patent Battles
     17.4 CRISPR-related Patentscape
     17.5 Patent Licensing
     17.6 It's Not All Over Yet
     References
Subject Index