Practical techniques in molecular biotechnology / Bal Ram Singh, Raj Kumar -- Cambridge : Cambridge University Press, 2021. – (58.2/S617)

Contents

List of Figures
List of Tables
Preface
1.  Introduction
    1.1  Biotechnology- Its Background and History
    1.2  Technology and Laboratory Practice
    1.3  Pedagogical Strategy of Biochemical Technology Practice
    1.4  Laboratory Safety
    1.5  Biosafety and Biosafety Levels
2. Recombinant DNA and Protein Technology
    2.1  Introduction
    2.2  Cloning
    2.3  Types of Cloning Vectors
    2.4  Expression Systems
    2.5  Promoters
    2.6  Protein Purification Methods
    2.7  Monitoring Protein Purification
3. Enzyme Kinetics, Proteomics, and Mass Spectrometry
    3.1  Order and Molecularity
    3.2  Important Theories Related to Enzyme Kinetics
    3.3  Enzymes
    3.4  Enzyme Kinetics
    3.5  Graphs of the Michaelis-Menten Equation
    3.6  Enzyme Inhibition
    3.7  Inhibitory Effect of Substrates
    3.8  General Protocol for an Inhibition Experiment
    3.9  Applications of Enzyme Inhibition
    3.10  Methodologies for Studying Catalytic Mechanism of the Enzyme
    3.11  Enzyme Activation
    3.12  Proteomics
    3.13  Mass Spectrometry
4. Bioanalytical Techniques
    4.1  Introduction
    4.2  Hydrodynamic Methods
    4.3  Biocalorimetry
    4.4  Spectroscopic Techniques Used on Biomolecules
    4.5  Circular Dichroism (CD)
    4.6  FTIR Spectroscopy
    4.7  Electrophoretic Techniques
5. Molecular Biology
    5.1  Introduction
    5.2  Structure of Nucleic Acids
    5.3  Functions of Nucleic Acids
    5.4  Genes and Genome
    5.5  Isolation and Separation of Nucleic Acids
    5.6  Manipulation and Detection of Nucleic Acids
    5.7  Polymerase Chain Reaction
    5.8  Nucleic Acid Sequencing
    5.9  Analyzing Gene and Gene Expression
    5.10  Protocols
6. Cell Culture
    6.1  Introduction
    6.2  Cell Structure and Function
    6.3  Cell Cycle
    6.4  Cell Microenvironment
    6.5  Primary Explantation versus Disaggregation
    6.6  Proliferation versus Differentiation
    6.7  Organotypic Culture
    6.8  Basics of Cell Culturing and Associated Measurements
    6.9  Propagation, Population Doubling and Passage Number
    6.10  Cell Viability
    6.11  Cryopreservation
    6.12  Characterization and Validation
    6.13  Microscopy
7. Antibody Technology
    7.1  Introduction to Immunochemical Techniques
    7.2  Antibodies
    7.3  Epitope Mapping
    7.4  Immunoassay
    7.5  ELISA
    7.6  Immunofluorescence
    7.7  Immunoblotting
    7.8  Immunoprecipitation Reaction
    7.9  Immunodiffusion
    7.10  Radioimmunoassay (RIA)
    7.11  Immunoelectrophoresis
    7.12  Immunosensors
    7.13  Immunotherapy
Appendices
    Appendix I  Troubleshooting: Cell Culture
    Appendix 2  Laboratory Safety
    Appendix 3  Statistics
    Appendix 4  Significant Figures
    Appendix 5  Units in the Biochemistry Laboratory
    Appendix 6  Chapter Contributors
    Appendix 7  Online Resources
Index
Color Plates