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Neutrophil : methods and protocols / edited by Mark T. Quinn, Frank R. DeLeo. -- Third edition. -- New York, NY : Humana Press, 2020. – (58.17/M592/v.2087) |
Contents
Dedication
Preface
Contributors
PART I NEUTROPHILS AND NEUTROPHIL DISORDERS: OVERVIEWS
1 The Role of Neutrophils in the Immune System: An Overview
2 Neutrophil Defects and Diagnosis Disorders of Neutrophil Function: An Overview
PART II NEUTROPHIL ISOLATION
3 Isolation of Human Neutrophils from Venous Blood
4 Isolation of Neutrophils from Nonhuman Species
5 Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Functional Studies
PART III NEUTROPHIL CHEMOTAXIS PHAGOCYTOSIS, AND BACTERICIDAL ACTIVITY
6 Analysis of Neutrophil Transmigration Through Epithelial Cell Monolayers
7 Quantification of Chemotaxis or Respiratory Burst Using Ex Vivo Culture-Derived Murine Neutrophils
8 Ex Vivo Human Neutrophil Swarming Against Live Microbial Targets
9 Microinjection and Micropipette-Controlled Phagocytosis Methods for Neutrophils
10 Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis
11 Visualization and Quantification of Phagocytosis by Neutrophils
12 Analysis of Neutrophil Bactericidal Activity
PART IV BIOCHEMISTRY, BIOLOGY, AND SIGNAL TRANSDUCTION OF NEUTROPHILS
13 Assessment of Neutrophil Apoptosis
14 Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils
15 Labeling Acidic Compartments of Neutrophils with Cresyl Violet
16 Neutrophil Degranulation of Azurophil and Specific Granules
17 Influence of Oxygen on Function and Cholesterol Composition of Murine Bone Marrow-Derived Neutrophils
18 In Vitro Assay for Sensitive Determination of Human Blood PMN Responses
19 Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR
20 Detection of Intact Transcription Factors in Human Neutrophils
21 Genome-Scale Transcript Analyses of Human Neutrophils
PART V NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES
22 Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes
23 Cell-Free NADPH Oxidase Activation Assays: A Triumph of Reductionism
PART VI ANALYSIS OF NEUTROPHIL EXTRACELLULAR TRAPS
24 Immunofluorescent Detection of NET Components n Paraffin-Embedded Tissue
25 Detection, Visualization, and Quantification of Neutrophil Extracellular Traps (NETs) and NET Markers
26 Imaging of Neutrophils and Neutrophil Extracellular Traps (NETs) with Intravital (In Vivo) Microscopy
Index
