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Methods in cell biology/ edited by Phong Tran. -- Cambridge, MA : Academic Press is an imprint of Elsevier, 2020. – (58.1574 /M592 /v.158) |
Contents
CHAPTER 1 A body-on-a-chip (BOC) system for
studying gut-liver interaction
1. Introduction
2. Materials
3. Methods
4. Final remarks
Acknowledgments
References
CHAPTER 2 Isolation of stage-specific germ
cells using FACS in Drosophila germarium
1. Introduction
2. Materials
3. Method
4. Notes
5. Conclusion
Acknowledgment
References
CHAPTER 3 How to orient cells in
microcavities for high resolution imaging of cytokinesis and lumen formation
1. Introduction
2. Design of mask for optimized cavity
preparation
3. Microfabrication of motifs on Si wafer
4. Process for cavity preparation
5. Choosing the optimal polymer for the
process: Advantages and drawbacks of different polymers
6. Cell seeding and visualization
7. Troubleshooting for cavity preparation
8. Conclusions
Acknowledgments
References
CHAPTER 4 Manipulating cultured mammalian
cells for mitosis research
1. Introduction
2. Cell culture
3. Imaging chambers
4. Microscope setup for live-cell imaging
5. Methods
Acknowledgment
References
CHAPTER 5 Live-cell FLIM-FRET using a
commercially available system
1. Introduction
2. Principles of FLIM-FRET
3. Equipment and materials
4. Proof of concept 1: CyclinB1-Cdk l
activity reporter in Drosophila $2 cells
5. Proof of concept 2: Camui CaMKII sensor
in human HEK293T cells
6. Utilizing the Nikon A1-FLIM platform
7. Conclusions
Acknowledgments
Conflicts of interest
References
CHAPTER 6 A comparative analysis of methods
to measure kinetochore-microtubule attachment stability
1. Introduction
2. Photoactivation of spindle microtubules
3. Nocodazole or cold shock assays
4. Calcium treatment assay
5. Interkinetochore distance and spindle
length measurements
6. Kinetochore oscillatory movement
7. Application of methods to evaluate the
effects of low dose nocodazole treatment
8. Concluding remarks
Acknowledgments
References
CHAPTER 7 Using STADIA to quantify dynamic instability
in microtubules
1. Introduction
2. Methods
3. STADIA outputs
4. Data analysis
5. Future developments
6. Conclusion
Acknowledgments
References
CHAPTER 8 Computational analysis of filament
polymerization dynamics in cytoskeletal networks
1. Introduction
2. Approach and rationale
3. Image analysis using ImageJ and Python
4. Conclusions
Acknowledgments
Competing interests
Author contributions
References
CHAPTER 9 A workflow for visualizing human
cancer biopsies using large-format electron microscopy
1. Introduction
2. Materials and methods
3. Conclusion
Acknowledgments
References
