Yeast protocols / edited by Wei Xiao. -- 4th ed. -- New York, NY : Humana Press, 2021. – (58.17 /M592 /v.2196)

Contents

Preface

Contributors

1 Use of Yeast Plasmids: Transformation and Inheritance Assays

2 Construction of Tight Conditional Mutants Using the Improved Auxin-Inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae

3 CRISPR Nickase-Mediated Base Editing in Yeast

4 Genomic Promoter Shuffling by Using Recyclable Cassettes

5 Study Essential Gene Functions by Plasmid Shuffling

6 Scarless Genomic Protein Labeling in Saccharomyces cerevisiae

7 High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae

8 Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections

9 Applications of, Oxford Nanopore Sequencing in Schizosaccharomyces pombe

10 Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map

11 Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly

12 Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE

13 Use Yeast Fab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering

14 A Versatile Protocol to Generate Translocations in Yeast Genomes Using CRISPR/Cas9

15 Yeast Nucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA

16 Assays for Autophagy III: Observing Dynamic Protein Trafficking

17 Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification

18 High-Throughput Rapid Yeast Chronological Lifespan Assay

19 Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast

20 Monitoring 5'-End Resection at Site-Specific Double-Strand Breaks by Southern Blot Analysis

Index