DNA vaccines : methods and protocols / edited by Ângela Sousa. -- New York : Humana Press, 2021. – (58.17 /M592 /v.2197) |
Contents
PART
I VECTOR DESIGN FOR VACCINATION
1 A Novel Cre Recombinase-Mediated In Vivo
Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application
2 Vaccination with Messenger RNA: A Promising
Alternative to DNA Vaccination
3 Impact of a Plasmid DNA-Based Alphavirus
Vaccine on Immunization Efficiency
PART
II DNA VACCINE ADJUVANTS AND IMMUNOSTIMULATORY
RESPONSE
4 CpG Oligonucleotides as Vaccine Adjuvants
5 Molecular Adjuvants for DNA Vaccines:
Application, Design, Preparation, and Formulation
6 Assessing Antigen-Specific Cellular Immune
Responses upon HIV/SIV Plasmid DNA Vaccination in the Nonhuman Primate Model
PART
III BIOTECHNOLOGICAL PROCESSES TO OBTAIN
DNA VACCINES
7 Enhanced Biosynthesis of Plasmid DNA from
Escherichia cell Applying Experimental Design
8 Primary Purification of Plasmid DNA Using
Differential Isopropanol Precipitation
9 Scale-Up of Plasmid DNA Downstream Process
Based on Chromatographic Monoliths
10 Purification of Plasmid DNA by Multimodal
Chromatography
11 Minicircle DNA Vaccine Purification and E7
Antigen Expression Assessment
PART
IV DNA VACCINE DELIVERY
12 Tumor-Specific CD8+ T-Cell Responses Induced
by DNA Vaccination.
13 Therapeutic DNA Vaccine Against
HPV16-Associated Cancer
14 Bulk and Microfluidic Synthesis of Stealth
and Cationic Liposomes for Gene Delivery Applications
15 Conception of Plasmid DNA and
Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines
Field
16 Main Features of DNA-Based Vectors for Use in
Lactic Acid Bacteria and Update Protocols
PART
V DNA VACCINE TRANSFER TO CLINICAL TRIALS
17 Ethics of DNA Vaccine Transfer for Clinical
Research
18 Preparation of an Academic Clinical Trial
Index